|
|
Zooplankton response to a change of water chemistry and amount of food during the recovery of lakes from acidification
Bartošíková, Martina ; Stuchlík, Evžen (advisor) ; Šorf, Michal (referee)
Anthropogenic acidification has affected assemblages in thousands of lakes in North America and Europe. It turned out that, during the acidification, the number of zooplankton changed and also the species composition of their assemblages, at some sites there was also the total disappearance. The main reason was a decrease of pH, wash out toxic aluminium and a change of trophic status. The rate and extent of biological recovery after restoring the pH in acidified lakes varies considerably across regions. Recovery of aquatic communities is significantly lagging behind the recovery in chemistry. Some zooplankton species are nonetheless able to quickly colonize recuperating ecosystems and in many acidified lakes again started to appear native species that have disappeared during acidification. The return of indigenous species, however, can be blocked acid-tolerant occurrence of species after species extinct occupied empty niches. Zooplankton can be affected by water chemistry directly, but also indirectly by the quality and quantity of food. An important factor is the possible spread of colonizing species. However, the relative roles of these factors in regulating recovery may be difficult to determine. Key words: zooplankton, acidification, water chemistry, aluminium toxicity, phytoplankton
|
|
|
The role of PLD in early phases of aluminium toxicity
Poláková, Lucie ; Schwarzerová, Kateřina (advisor) ; Malínská, Kateřina (referee)
Aluminium toxicity is the main limiting factor in crop production on acid soils. The main symptom of aluminium toxicity is a rapid inhibition of root growth, but the mechanism of root growth cessation remains unclear. In this diploma thesis we deal with the question of whether phospholipases PLDα1 and PLDδ may play a role in the mechanism of aluminium toxicity. We compared the responses of plants lacking PLDα and PLDδ with WT plants. Growth analysis of roots was performed in hydroponic conditions. The most sensitive part of roots was transient zone in which cells were dying earlier. It was further found that pldα1 plants were less sensitive on aluminium toxicity because their roots showed less growth inhibition than WT. Pldδ plants did not differ from WT plants in their response to aluminum. During further analysis of the pldα1 reactions, it was found that the root cells were capable of cell expansion during aluminum toxicity, and the cellular malformations were formed on the roots. This phenomenon was associated with faster reorientation and even depolymerization of cortical microtubules in response to toxic aluminium in pldα plants compared to WT plants. The results indicated that PLDα1 molecule affects the stability of cortical microtubules. Microtubules were less stable and they depolymerized...
|
guest ::
